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Aquarium Answers, Pond Fish Question and Help Articles

aquarium answers pond fish question and help articles
The drilled cylindrical muscle was inserted into a 5 mm NMR tube filled with 100 μl of KPi 0. 1 M K2HPO4/KH2PO4 in D2O, pH 7. 0 with 1 mM of sodium 2,2 dimethyl 2 silapentane 5 sulfonate DSS as an inner average and centrifuged at 3000 rpm for 10 min for degasification. For NMR observations of metabolites and major macromolecules, the ultimate fish muscle was lyophilized and beaten into powder. The supernatant with 1 mM of DSS was transferred to a 5 mm NMR tube. For metabolome observations, two dimensional J resolved 2DJ spectra pulse series of jresgpprqf were obtained at 298 K using a Bruker AVANCE II 700 spectrometer capable with a 1H inverse triple resonance cryogenically cooled probe with Z axis gradients Bruker BioSpin GmbH, Rheinstetten, Germany.