siamese fighting fish behaviourGene expression was studied by RT PCR. Total RNA from unbiased tissues or embryos was isolated using TRIzol reagent Invitrogen. Two micrograms of total RNA were handled with DNase I Roche after which used for cDNA synthesis using Reverse Transcriptase M MLV Promega. Expression of genes of attention in alternative tissues and in different developmental stages was in the beginning tested using RT PCR with gene true primers. The relative expression of candidate genes was then studied using real time RT PCR qRT PCR using KAPA SYBR FAST qPCR Kits KapaBiosystems with CFX96 Touch Real Time PCR Detection System Bio Rad. Three replicates were carried out for every sample and cDNA from 50 ng of total RNA was used for each reaction.