The genome size of the fighting fish was estimated in response to k mer frequencies, de novo RADtags mapping and Q PCR method supplementary note 1, Supplementary Material online. Completeness of the genome meeting was evaluated using BUSCO Simão et al. 2015 and by the mapping rate of transcripts and de novo RADtags. Three mRNA libraries were one at a time built for one male and two females of 3 months’ age. Total RNA was remoted from brain, eye, skin, gill, muscle, gut, spleen, liver, heart, kidney, and gonad, after which equal amounts from each tissue were pooled for library development using Illumina TruSeq RNA sample guidance kit Illumina. Moreover, RNA samples of an alternate mature male and female, derived from the above pooling strategy, were used for total RNA library construction with rRNA depletion, using NEBNext Ultra RNA Library Prep Kit NEB.