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Goldfish Bowl?

goldfish bowl
The outer primers were utilized in the first round of PCR to prepare the average templates in the second round of real time PCR. During here round of real time PCR with the inner primers, ordinary curves were drawn using diluted usual templates of different concentrations. Then the genomic DNA of target species was used as template in the real time PCR with the above volume and protocol. We compared the amplification curve with the normal curves to estimate after which calculated the genome size in response to the quantified awareness and copies. The performance details were defined in Supplementary Method. QTL mapping evaluation was conducted for 2 growth traits, BW and SL, using GridQTL with the regression interval mapping method37.