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Introduction — fish shell . . documentation

introduction fish shell documentation
Slides are washed, dried and then immersed in blocking off buffer 1X PBS, 0. 1 % Triton 100 for 2 min, and rinsed in PBS for 5 min at room temperature. After washing, 60 μl of an antifade answer p phenylenediamine 10 mg/ml, 90 % glycerol, and propidium iodide 1 μg/ml as a counterstain is added on each slide. The slide is observed with fluorescence microscopy using B2 or B 2A filter cassette. COMBO FISH is used for precise labeling of genomic sites. It takes advantage of homopurine/homopyrimidine oligonucleotides that form triple helices with intact duplex genomic DNA.