siamese fighting fish staying at top of tank1999, 2001. To verify even if mitfa is associated with the albino phenotype, we knocked out this gene using CRISPR/Cas9 system by concentrated on the coding sequences supplementary fig. S12, Supplementary Material online. Two G0 CRISPants with out detectable wild type alleles absolutely lost melanin pigmentation, while the mosaic fish with both wild type and mutated haplotypes were markedly decreased in the density of melanin containing cells, in comparison with wild type controls, which invariably offered normal melanin pigmentation 48 hpf fig. 2F and supplementary fig. S13, Supplementary Material online.