siamese fighting fish online uk2009 using Gateway Recombination Cloning Technology Thermo Fisher Scientific. T7 Transposase Khattak et al. 2014 Addgene was transcribed using mMESSAGE mMACHINE T7 kit Life Technologies, based on the manufacturer’s instructions. A final attention of 40 ng/µl ZED constructs, 50 ng/µl transposase mRNA, and 0. 05% phenol red were coinjected into one cell stage embryos. The embryos were imaged for GFP and inner control red fluorescent protein RFP expression at alternative time points using a Leica MZFLIII microscope.