why is my siamese fighting fish losing colourrerio, medaka O. latipes, stickleback G. aculeatus, fugu T. rubripes, and Nile tilapia O. niloticus downloaded from Ensembl database, unencumber 86 using LASTZ Harris 2007. Multiple alignments were generated with MULTIZ Blanchette et al. The sequences were softmasked using RepeatMasker Chen 2004 according to the repeat libraries got from RepeatModeler , Repbase Jurka et al. 2005, and MAKER Cantarel et al. 2008 series repeat databases. Both evidence based and ab initio gene models were used for annotation. Transcriptomes of fighting fish and protein sequences of zebrafish, medaka, stickleback, fugu, and Nile tilapia from Ensembl database unlock 86 were used for proof. SNAP Korf 2004 and Augustus Stanke and Waack 2003 were iteratively used for ab initio gene models training.