D PCR screening of the deletion in single tail and double tail fish. E Representative fighting fish injected with enhancer detection vector ZED built with CNE. 006008 from single tail allele ST appearing GFP expression predominantly in the dorsal fin and caudal fin positions, and those injected with double tail allele st showing no GFP expression in the whole embryos at 24 hpf. RFP that is barely detectable, particularly in muscle tissue, since 72 hpf, is used as internal handle. F Relative luciferase exercise in Singapore grouper embryonic cell line transfected with pGL3 Promoter constructs including CNE. 001.