2018; Hofstetter et al. 2019. Mutations in coding sequences usually tend to alter protein functions. In certain for pleiotropic factors, such mutations may be more harmful to the organisms than those occurring in regulatory regions, which only affect the expression level Wittkopp and Kalay 2012; Petit et al. 2017. In this regard, mutations in the coding sequences of microphthalmia associated transcription elements have been appreciably said to cause a variety of defects in animals Tassabehji et al.