marina betta siamese fighting fish aquarium kitFixed or nearly fixed variations were annotated and protein coding genes within this locus were personally analyzed by literature mining. Genes associated with fin advancement and regeneration were kept for expression analysis using real time reverse transcription PCR RT PCR to determine candidate genes. In order to simply differentiate among genotypes, we developed indel markers for instant PCR assays for double tail and albino traits. The shortest genomic region due to arrangement mapping was used for marker screening. Homozygous americans with regard to the above traits were resequenced for marker discovery using GATK pipeline DePristo et al. 2011 in keeping with our old study Wang et al.