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siamese fighting fish tank conditions

siamese fighting fish tank conditions
Information about animalssiamese fighting fish sickThree replicates were conducted for every sample and cDNA from 50 ng of total RNA was used for each response. Beta actin or EF1A was used as endogenous reference based on their expression balance. Candidate enhancer regions with intently flanking sequences from alternative alleles were cloned and constructed into the enhancer region of pGL3 Promoter vector that comprises a basal SV40 promoter series Promega. The reporter gene constructs along with pRL Renilla Luciferase Control Reporter Vector Promega were cotransfected into a Singapore grouper embryonic cell line Chew Lim et al. 1994 using TurboFect or Lipofectamine 3000 Thermo Fisher. Luciferase activity was measured at 48 h post transfection using Dual Luciferase Reporter Assay System Promega.