siamese fighting fish swim bladder disease006008 of double tail allele. D PCR screening of the deletion in single tail and double tail fish. E Representative fighting fish injected with enhancer detection vector ZED constructed with CNE. 006008 from single tail allele ST showing GFP expression predominantly in the dorsal fin and caudal fin positions, and those injected with double tail allele st showing no GFP expression in the complete embryos at 24 hpf. RFP that is just detectable, particularly in muscle mass, since 72 hpf, is used as inner management. F Relative luciferase pastime in Singapore grouper embryonic cell line transfected with pGL3 Promoter constructs adding CNE.