Completeness of the genome meeting was evaluated using BUSCO Simão et al. 2015 and by the mapping rate of transcripts and de novo RADtags. Three mRNA libraries were separately constructed for one male and two women folk of 3 months’ age. Total RNA was remoted from brain, eye, skin, gill, muscle, gut, spleen, liver, heart, kidney, and gonad, after which equal quantities from each tissue were pooled for library development using Illumina TruSeq RNA sample guidance kit Illumina. Moreover, RNA samples of another mature male and feminine, derived from the above pooling approach, were used for total RNA library construction with rRNA depletion, using NEBNext Ultra RNA Library Prep Kit NEB. Raw sequences were wiped clean with manner shortreads r c q t 150 in Stacks package Catchen et al.